Based on our results, Harmony, LIGER, and Seurat 3 are the recommended methods for batch integration. Due to its significantly shorter runtime, Harmony is recommended as the first method to try, with the other methods as viable alternatives. Nature Benchmarking single cell RNA-sequencing analysis - Nature.
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Pro.digbi.net · mod · resourcepro.digbi.net. The advent of single-cell RNA sequencing (scRNA-seq) technologies has revolutionized transcriptomic studies. However, large-scale integrative analysis of scRNA-seq data remains a challenge largely. Mar 25, 2021 · In many biological applications of single-cell RNA sequencing (scRNA-seq), an integrated analysis of data from multiple batches or studies is necessary. Current methods typically achieve. Learning interpretable cellular and gene signature embeddings. Www.nature.com · articles · s42256/021/00361-8Integration of millions of transcriptomes using batch-aware. Jan 16, 2020 · Comparing the iLISI scores, scMerge was the top method for batch mixing, and LIGER was a close second (p = 0.015) (Fig. 3). All methods gave good cLISI scores (1-cLISI 0.96), which is congruent with the visualizations. For kBET, Harmony was top for batch mixing, followed by LIGER. 007toto. and scGen (p 0.001).
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